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INVESTIGACIÓN

Test-retest reliability of the human functional
connectome over consecutive days: identifying
highly reliable portions and assessing the impact
of methodological choices

Leonardo Tozzi1, Scott L. Fleming2, Zachary D. Taylor3, Cooper D. Raterink3,

and Leanne M. williams

1Department of Psychiatry and Behavioral Sciences, Universidad Stanford, stanford, California, EE.UU
2Department of Biomedical Data Science, Universidad Stanford, stanford, California, EE.UU
3Departamento de Ciencias de la Computación, Universidad Stanford, stanford, California 94305, EE.UU

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diario

Palabras clave: resonancia magnética funcional, Conectividad funcional, Resting state, Reliability

ABSTRACTO

Countless studies have advanced our understanding of the human brain and its organization
by using functional magnetic resonance imaging (resonancia magnética funcional) to derive network representations of
human brain function. Sin embargo, we do not know to what extent these “functional
connectomes” are reliable over time. In a large public sample of healthy participants
(norte = 833) scanned on two consecutive days, we assessed the test-retest reliability of fMRI
functional connectivity and the consequences on reliability of three common sources of
variation in analysis workﬂows: atlas choice, global signal regression, and thresholding.
By adopting the intraclass correlation coefﬁcient as a metric, we demonstrate that only a
small portion of the functional connectome is characterized by good (6–8%) to excellent
(0.08–0.14%) fiabilidad. Connectivity between prefrontal, parietal, and temporal areas is
especially reliable, but also average connectivity within known networks has good reliability.
En general, while unreliable edges are weak, reliable edges are not necessarily strong.
Methodologically, reliability of edges varies between atlases, global signal regression
decreases reliability for networks and most edges (but increases it for some), and thresholding
based on connection strength reduces reliability. Focusing on the reliable portion of the
connectome could help quantify brain trait-like features and investigate individual
differences using functional neuroimaging.

RESUMEN DEL AUTOR

We quantiﬁed the reliability of fMRI functional connectivity in a large sample of healthy
Participantes (norte = 833) scanned over two consecutive days. We also assessed the
consequences on reliability of atlas choice, global signal regression and thresholding. Only a
small portion of the functional connectome has good (6−8%) to excellent (0.08−0.14%)
fiabilidad. Connectivity between prefrontal, parietal and temporal areas is especially reliable
and average connectivity within known networks has good reliability. While unreliable edges
are generally weak, reliable edges are not necessarily strong. Reliability of edges varies
between atlases. Global signal regression decreases reliability for networks and most edges
(but increases it for some). Thresholding based on connection strength reduces reliability.
Focusing on reliable portions of the connectome could help investigate individual differences
using functional neuroimaging.

Citación: Tozzi, l., Fleming, S. l.,
taylor, z. D., Raterink, C. D., &
williams, l. METRO. (2020). Test-retest
reliability of the human functional
connectome over consecutive days:
identifying highly reliable portions
and assessing the impact of
methodological choices. Red
Neurociencia, 4(3), 925–945.
https://doi.org/10.1162/netn_a_00148

DOI:
https://doi.org/10.1162/netn_a_00148

Supporting Information:
https://doi.org/10.1162/netn_a_00148
https://db.humanconnectome.org/
https://github.com/leotozzi88/
reliability_study

Recibió: 27 Febrero 2020
Aceptado: 13 Puede 2020

Conflicto de intereses: Los autores tienen
declaró que no hay intereses en competencia
existir.

Autor correspondiente:
Leanne Maree Williams
leawilliams@stanford.edu

Editor de manejo:
Lucina Uddin

Derechos de autor: © 2020
Instituto de Tecnología de Massachusetts
Publicado bajo Creative Commons
Atribución 4.0 Internacional
(CC POR 4.0) licencia

La prensa del MIT

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Human functional connectome reliability

Functional connectome:
The ensemble of the functional
connectivity values between all
regions in a brain.

Reliability:
We consider a measure “reliable”
when it features high
between-subjects variability, coupled
with low within-subject variability.

Conectividad funcional:
Pearson correlation coefﬁcient
calculated between two
blood-oxygen-level dependent
(BOLD) timeseries derived from two
regiones del cerebro.

Functional magnetic resonance
imaging (resonancia magnética funcional):
A technology that indirectly
measures brain activity by detecting
changes in the blood-oxygen-level
dependent (BOLD) contrast in a
magnetic resonance scanner.

INTRODUCCIÓN

The human brain is an extraordinarily complex network comprising one hundred billion neu-
ron, each connected to an average of 7,000 other neurons. This yields between 100 trillion
y 1 quadrillion synapses, depending on a person’s age (Drachman, 2005). Current research
in neuroscience suggests that it is the architecture and dynamic interactions of neurons that
give rise to complex phenomena, such as cognition and emotion (bassett & despreciar, 2017;
Lindquist et al., 2012; Mill et al., 2017). This has been called the “functional connectome,"
and over the past three decades, several studies have characterized it in vivo (ver, Por ejemplo,
Van Essen et al., 2013). There is consensus that we need to map the human brain connectome
in order to move forward our fundamental understanding of the human brain and its organi-
zación. Sin embargo, we do not yet know to what extent the functional connectome is stable over
time for an individual. In the present work, our aim is to explore the short-term reliability (en
the order of days) of functional connectomes. We believe this is a fundamental step toward the
identiﬁcation of a persistent representation of brain function, which will facilitate the mapping
of cognitive processes in individuals and will be critical for linking connectivity disruptions to
brain disorders.

Over the past three decades, conectividad funcional (FC) has become a well-established
approach to measuring the functional connectome by using functional magnetic resonance
imaging (resonancia magnética funcional). The term FC refers to synchronous distributed ﬂuctuations in neuronal activity
and is thought to represent a correlate of the dynamic interaction between neurons located
in different brain areas (Lowe et al., 2000). In most studies, FC is measured by computing a
Pearson correlation of the fMRI-derived blood oxygen level–dependent (BOLD) time series of a
set of regions while the participant is awake and not performing any task (Lowe et al., 2016).
This is what we will intend as FC in the present work, but it is important to note that FC
can also be computed while participants are not completely idle and by using a wide array
of methods besides correlations, such as independent component analysis, analyses in the
frequency domain, Bayesian models, and dynamic approaches (for review, Gonzalez-Castillo
& Bandettini, 2018; Lowe et al., 2016; Preti et al., 2017; Smitha et al., 2017).

En años recientes, FC has been used to answer important questions about both healthy and
disordered brain function. Por ejemplo, psychiatry and neurology have turned to FC to de-
velop new diagnostic tests, predict treatment response, and relate brain function to symptoms
(Fornito et al., 2015; Sha et al., 2018). This is in answer to an urgent need for quantitative
correlates of brain illnesses, and network-based approaches show great promise to this end
(williams, 2017). Sin embargo, when testing candidate measures for clinical applications, soy yo-
portant to consider that measures of robust group-level effects, which make up a large portion
of the existent literature, might not necessarily be suited to make inferences about individuals
(Hedge et al., 2018). Por ejemplo, a network might show consistent FC values because of its
low between-subjects variability, but this same characteristic would make it unsuitable to in-
vestigate its correlations with measures that might be highly variable between subjects (symp-
toms, personality, etc.). También, one reasonable characteristic of a measure considered for clinical
applications is repeatability: the same test on the same individual after a short period of time
should return very similar results (Sullivan et al., 2015). In the present work, we deﬁne reliabil-
ity as the combination of high between-subjects variability, coupled with low within-subject
variabilidad, a combination that is advantageous when trying to relate biomarkers to individual
traits (Fleiss et al., 2003; Zuo et al., 2019b). Desafortunadamente, several of the measurements
commonly collected by researchers to relate brain function to behavior have poor reliability
(Hedge et al., 2018), which might explain their weak relationship to trait-like features (ver, para
It follows that for any study investigating individual
ejemplo, Eisenberg et al., 2019).

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Human functional connectome reliability

differences using neuroimaging, ensuring the reliability of measurements is of paramount im-
portance, even outside of the realm of clinical applications (Xing & Zuo, 2018; Zuo et al.,
2019a, 2019b).

Hasta la fecha, only a relatively small number of studies has examined in detail the reliability of
the functional connectome, summarized by two recent papers (Noble et al., 2019; Zuo & Xing,
2014). There is consensus that connectomes tend to become more reliable the longer the du-
ration of the scan (Anderson et al., 2011; Birn et al., 2013; Elliott et al., 2019; Noble et al.,
2019; Termenon et al., 2016). Sin embargo, which and how many functional connections can
be measured reliably is unclear. Meta-analytical evidence shows that, on average, FC relia-
bility is poor (Noble et al., 2019). Some studies, sin embargo, report that functional connections
have “fair” reliability on average (as deﬁned by Cicchetti, 1994) but others report “good” or
“excellent” reliability for large (>25%) portions of the functional connectome, in particular of
well-characterized functional networks such as the default mode, fronto-parietal, and dorsal
attention networks (Birn et al., 2013; Elliott et al., 2019; Guo et al., 2012; Zuo & Xing, 2014).
Reliability of global and local graph metrics derived from functional connectomes has also
been shown to only be in the “fair” range, but can still be considered statistically signiﬁcant
(Termenon et al., 2016). One reason for these discrepancies might be that most studies only
used relatively small samples (norte <= 50) and had long intervals between scanning sessions (days or months). Sample size has been found to affect reliability estimates (Termenon et al., 2016), and shorter time intervals are better suited for measuring test-retest reliability, since they minimize the variability introduced by ancillary factors (Sullivan et al., 2015). Also, there are several choices that are routinely made when computing functional connectomes and it is unclear how these might affect their reliability. Examining all such possible choices is beyond the possibilities of the current work, but we focus here on three procedures for which a clear guideline in the community is lacking. The ﬁrst is the set of regions (atlas) used to compute FC. Choosing atlases with a higher number of regions has been shown to increase reliability (Termenon et al., 2016), but it is unclear if a consensual pattern of reliable connections exists across atlases. Second, global signal regression term (GSR) is often used as a denoising pro- cedure but potentially affects FC and has been suggested to decrease reliability (Elliott et al., 2019; Murphy & Fox, 2017; Power et al., 2018). Third, to compute graph metrics based on the functional connectome that require sparsity, usually weaker connections are removed, either based on an absolute or relative threshold, that is, edges below a certain value, or the bottom nth percentile. Different graph metrics appear to have highest reliability at different thresholds (Termenon et al., 2016). It is, however, unclear if edge strength and reliability are related, how thresholding affects the reliability of edges, and even if the same edges are retained when functional connectomes from the same individual are thresholded independently. In the present work, we explore short-term test-retest reliability of functional connectomes in a very large sample of healthy individuals scanned on two consecutive days. In particular, we leverage the entire Human Connectome Project (HCP) Healthy Young Adult data release. This dataset makes use of cutting-edge acquisition and preprocessing techniques, has very long resting-state sessions (30 min), is publicly available, and is a widely used gold standard In these ideal conditions, ﬁrst, we assess for transparent and reproducible methods testing. reliability of the edges and known networks that make up the functional connectome. Then, we examine the impact on reliability of atlas choice, GSR, and thresholding. MATERIALS AND METHODS All scripts to reproduce our analyses and plots are available on GitHub at https://github.com/ leotozzi88/reliability_study. The data processing ﬂow is shown in Supporting Information Atlas: A set of labels covering the entire brain by which every grayordinate is assigned to a speciﬁc brain region. Global signal regression: A preprocessing practice that involves ﬁtting a linear regression model predicting the timeseries of a region by using as a predictor the average timeseries of all regions. The residuals of this model are then retained for further analysis. Network Neuroscience 927 l D o w n o a d e d f r o m h t t p : / / d i r e c t . m i t . / / t e d u n e n a r t i c e - p d l f / / / / / 4 3 9 2 5 1 9 2 0 7 6 6 n e n _ a _ 0 0 1 4 8 p d . t f b y g u e s t t o n 0 7 S e p e m b e r 2 0 2 3 Human functional connectome reliability Figure S1, along with the name of the scripts in this repository corresponding to each anal- ysis step. Dataset Our sample is derived from the HCP Healthy Young Adult release, a large public dataset of 1,200 subjects aged between 22 and 35 years without any psychiatric or neurological disor- der (Van Essen et al., 2013). The acquisition parameters and minimal preprocessing of these data are described in Glasser et al. (2013). Brieﬂy, participants underwent a large number of MRI scans, that included T1- and T2-weighted structural imaging, diffusion tensor imaging, and nearly 2 hours of resting-state and task multiband fMRI. For the present study, we used 1 hour of resting-state fMRI collected on each participant during four 15-min scans (1,200 time points each, two runs acquired with RL phase encoding and two with LR) split in two scanning sessions over two days. To select our sample, we accessed the data at https://db.humanconnectome.org. Using the online ﬁltering options, we selected only participants who had completed the full resting-state scanning protocol and had no known quality issues. This returned a total of 860 subjects, each with four resting-state fMRI runs. For these, we downloaded the framewise relative root-mean- square realignment estimates (RMS) and fMRI data denoised using ICA-FIX (Salimi-Khorshidi et al., 2014). All analyses were conducted in greyordinate space, that is, they were constrained to the gray matter by using ﬁles in the CIFTI format, thus taking full advantage of HCP prepro- cessing and minimizing nonneuronal signal (Glasser et al., 2013). Connectivity Matrix Construction Each dense denoised timeseries resting-state ﬁle was parcellated using connectome workbench (wb_command-cifti-parcellate) to obtain the mean timeseries in each atlas region (see below). All further analyses were conducted in MATLAB R2018a (9.4.0.949201) for Mac (The Math- Works, Inc.). For each subject, parcellated timeseries as well as framewise RMS were loaded. Then, GSR was performed (see below). A high-pass ﬁlter (0.008-Hz cut-off) was applied to the timeseries. High frequencies were retained to avoid excessive loss of degrees of freedom due to the very low TR (Bright et al., 2017). Volumes with RMS > 0.30 were ﬂagged as containing
motion and were removed from the timeseries (Power et al., 2014). We also excluded any
subject for which volumes ﬂagged for motion exceeded 15% in any of the four resting-state
carreras. This step resulted in a ﬁnal sample size of 833. The two runs within each one of the two
sessions were then concatenated, and Pearson correlation between all the timeseries was used
to obtain a connectivity matrix for each session. At the end of this procedure, each subject
had six matrices (3 atlases, with and without GSR) for each of two sessions, for a total of 9,996
connectivity matrices.

Connectivity of Established Resting-State Networks

From each connectivity matrix, the average FC within each of 12 established resting-state
networks was computed using the labels of the Gordon Atlas (Gordon et al., 2016). Estos
resting-state networks were default mode, parieto-occipital, fronto-parietal, prominencia, cingulo-
opercular, medial-parietal, atención dorsal, atención ventral, visual, supplementary motor
(mano), supplementary motor (mouth), and auditory. The reliability of these aggregate network
statistics was then assessed.

Resting state:
A paradigm in which blood-
oxygen-level dependent (BOLD)
signal is recorded in the absence
of any explicit task.

Connectivity matrix:
A symmetric matrix with a diagonal
of ones representing the functional
connectivity values between all
regiones del cerebro.

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Human functional connectome reliability

Test-Retest Reliability

Intraclass correlation coefﬁcient (CPI) as implemented in MATLAB (https://www.mathworks.
com/matlabcentral/ﬁleexchange/22099-intraclass-correlation-coefﬁcient-icc) was used to quan-
tify the test-retest reliability of FC. En particular, we assessed the consistency among measure-
ments under the ﬁxed levels of the session factor, in line with previous work (Chen et al., 2018;
Elliott et al., 2019). This measure has been named ICC ‘C-1’ in McGraw and Wong (1996) o
CPI (3,1) in Shrout and Fleiss (1979).

To calculate ICC for all our FC values, primero, the FC values in the upper triangle of each
subject’s connectivity matrix were entered as rows in two large matrices (one matrix for each
session, one row per subject in each matrix). Entonces, the corresponding columns of these ma-
trices were compared to obtain an ICC value. Since the number of features (and thus ICCs)
was very large (de 21,945 a 61,776 depending on atlas), we report the median, minimum,
and maximum ICC as well as the portion of functional connections having poor (<0.40), fair (0.40–0.60), good (0.60–0.75), or excellent (>0.75) ICC as deﬁned by Cicchetti (1994).

This procedure was conducted for connectivity matrices obtained using all atlases, with and

without GSR as well as for the resting-state networks average FC.

Effects of Atlas on Reliability

For each subject and each session, we computed functional connectomes by using three atlases
that are widely used within the neuroimaging community and available in CIFTI format. El
ﬁrst is the Brainnettome Atlas, derived by structural and FC (Fan et al., 2016). The second is the
Glasser Atlas, based on the multimodal cortical parcellation of HCP participants (Glasser et al.,
2016). The third is the Gordon Atlas, which is based on resting-state FC and provides labels
identifying well-established resting-state networks (Gordon et al., 2016). Since none of three
atlases includes subcortical structures, these were derived from the Freesurfer segmentation
(Fischl et al., 2002) and added to each CIFTI dense label ﬁle using connectome workbench
(wb_command-cifti-create-dense-from-template). To test for a difference in ICC values across
atlases, we used a Kruskal–Wallis test.

Effect of Global Signal Regression on Reliability

Grayordinate:
A brain gray matter location that can
be represented by a surface vertex
(nodo) or a volume voxel.

Immediately after loading the timeseries data in MATLAB, the mean of the grayordinate time-
series from all regions was regressed from each timeseries to produce a set of GSR-corrected
timeseries (Burgess et al., 2016). Analyses then proceeded in the same way separately for GSR-
corregido (GSR+) and noncorrected (GSR−) timeseries. To test for a difference in ICC values
computed using GSR+ and GSR− timeseries, we used a Wilcoxon signed rank test.

Effect of Edge Strength on Reliability

To get a measure of edge strength, we averaged the FC of all edges across the two sessions and
across all subjects. Entonces, for each atlas, we computed a Spearman correlation between edge
strength and ICC of the edge calculated as outlined above.

Effects of Thresholding on Reliability

To test the effects of thresholding the functional connectome on reliability estimates and if
the same edges would be consistently retained across sessions, we proceeded as follows.
Primero, we deﬁned 20 evenly spaced threshold values from 0.05 a 1. For each value and each

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Human functional connectome reliability

connectivity matrix, two new matrices were created using functions from the brain connec-
tivity toolbox (Rubinov & despreciar, 2010). In the ﬁrst matrix, all FC values below the threshold
were set to 0 (absolute threshold). In the second, the proportion of strongest FC values cor-
responding to the threshold was retained (relative threshold). Entonces, for each absolute and
relative threshold, we examined all edges that were retained at least in one session. We com-
puted the ratio between the number of participants in which each edge was retained at both
time points versus the ones in which it was retained at least once. This quantity, which we
name “ratio of consistent edges,” or “consistency ratio” for short, goes from 0 (each time the
edge is retained, it is only retained in one session) a 1 (each time the edge is retained, es
retained in both sessions). We report the median, minimum, and maximum ratio of consistent
edges for each threshold and atlas as well as the proportion of poor (<0.40), fair (0.40–0.60), good (0.60–0.75), or excellent (>0.75) edges using the same cutoffs as for ICC for convenience
(Cicchetti, 1994). We also tested whether the median ratio of consistent edges was correlated
with the threshold value by using a Spearman correlation. Finalmente, for each absolute and rela-
tive threshold, we computed the ICC as described above only in subjects for which the edge
was retained in both sessions, so as not to bias our calculation by the edge potentially being
set to 0 because of thresholding in one session. We also computed whether median ICC values
were correlated with the threshold value by using a Spearman correlation.

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Conﬁrmation of Results in Nonrelated Subsample

Since several subjects in the Healthy Young Adult dataset share family membership and
a signiﬁcant portion of variance in FC is explained by genetics (Adhikari et al., 2018; eliot
et al., 2019; Reineberg et al., 2018), we reran our analyses on an unrelated subset of our
dataset to conﬁrm our results (norte = 397 after accounting for data availability and motion).

Conﬁrmation of Results Using Different ICC Intervals

Since the cutoffs reported in Cicchetti (1994) are just one of the possible ways to classify ICC
valores, we reran our analyses using a more ﬁne-grained binning scheme with ﬁve instead of
four classes: slight (<0.20), fair (0.20–0.40), moderate (0.40–0.60), substantial (0.60–0.80), and perfect (>0.80) (Xing & Zuo, 2018).

RESULTADOS

Test-Retest Reliability of the Functional Connectome

Regardless of atlas and without performing GSR (see below), the majority of edges of the func-
tional connectome were in the “fair” reliability range (Cifra 1).

Median ICC ranged from 0.41 a 0.47 depending on atlas (Mesa 1 y figura 1). Given
the large sample size, estimation of ICC was accurate: the width of conﬁdence intervals for
ICC in all three atlases varied between 0.04 y 0.14 and had a median of 0.10 (Secundario
Information Figure S2). When examining the average ICC of all edges touching each node,
the subgenual anterior cingulate cortex and inferior temporal lobe had the lowest average
fiabilidad. Average ICC was also low in areas immediately adjacent to the corpus callosum
(cingulate cortex). The most reliable nodes on average were located in the superior parietal
and middle temporal lobes (Cifra 2).

“Good” edges always represented a relatively small portion compared to the total (6–8%)
but still numbered in the thousands. These connections mostly involved the inferior parietal

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Human functional connectome reliability

Cifra 1. Reliability of functional connectome edges. Left: For the Brainnetome, vidrio, y
Gordon atlases with and without performing GSR, we show boxplots of ICC of all atlas edges. Right:
For the Brainnetome, vidrio, and Gordon atlases with and without performing GSR, we show the
proportion of edges having poor (CPI < 0.40), fair (ICC = 0.40–0.60), good (ICC = 0.60–0.75), or excellent (ICC > 0.75) fiabilidad, deﬁned in accordance to Cicchetti (1994). GSR− = no global
signal regression; GSR+ = global signal regression; ICC = intraclass correlation coefﬁcient.

Mesa 1. Reliability of edges in the functional connectome.

Brainnetome

vidrio

gordon

Median
CPI

0.4688

0.3868

0.4587
0.3522

0.4122

0.3113

mín.
CPI
−0.0682
−0.0750
−0.0627
−0.0889
−0.0845
−0.0965

máx.
CPI

0.8145

0.8713

0.8277
0.8643

0.8102

0.8453

GSR−
GSR+
GSR−
GSR+
GSR−
GSR+

Poor edges
ratio

Fair edges
ratio

Good edges
ratio

Excellent edges
ratio

0.2884

0.5294

0.3085
0.6143

0.4663

0.6939

0.6271

0.3328

0.6124
0.3103

0.4755

0.2361

0.0832

0.1191

0.0777
0.0714

0.0574

0.0663

0.0014

0.0187

0.0014
0.0040

0.0008

0.0037

Nota. We show the median, minimum, and maximum ICC of functional connectomes computed using three different atlases, con
or without global signal regression. We also show the proportion of edges having poor (CPI < 0.40), fair (ICC = 0.40–0.60), good (ICC = 0.60–0.75), or excellent (ICC > 0.75) fiabilidad, deﬁned in accordance to Cicchetti (1994).
ICC = intraclass correlation
coefﬁcient; GSR− = no global signal regression; GSR+ = global signal regression.

and middle temporal lobes, but were also present in the frontal, superior parietal, and occipital
lobes (Cifra 3). “Excellent” edges were relatively rare and consistently less than a hundred
(0.08–0.14% of total connections). These were mostly intrahemispherical and predominantly
connected the prefrontal, parietal, and temporal lobes (Cifra 4).

Test-Retest Reliability of Resting-State Networks

Average FC within established resting-state networks always had “good” reliability. The most
reliable network was the parieto-occipital (ICC = 0.73), followed by the medial-parietal

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Human functional connectome reliability

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Cifra 2. Average ICC of functional connections by node. On an inﬂated brain we show, for each
node of the Brainnetome, vidrio, and Gordon atlases, the average ICC of all functional connections
involving the node. ICC = intraclass correlation coefﬁcient.

(ICC = 0.71), and auditory (ICC = 0.71). The least reliable were the salience (ICC = 0.63),
atención dorsal (ICC = 0.64), and supplementary motor (mouth) (ICC = 0.64) (Cifra 5 y
Mesa 2).

Effects of Atlas on Reliability
ICC differed signiﬁcantly between atlases (Kruskal–Wallis chi-square = 4.96 × 103
, pag < 1 × 10−32 ). The Brainnetome and Glasser atlases had comparable median ICCs (0.47 and 0.46, respectively; Table 1). The proportion of “good” and “excellent” edges was also comparable (8% and 0.14% for both atlases, respectively). However, since the Glasser Atlas has more nodes than the Brainnetome (229 vs. 379), it generated a much larger number of edges (71,631 vs. 26,106). Therefore, using the Glasser Atlas returned a higher absolute number of “good” and “excellent” edges (Figure 4). Median ICC for the Gordon Atlas (352 nodes) was lower (0.41), as well as the proportion of “good” (5%) and “excellent” (0.08%) edges. Network Neuroscience 932 Human functional connectome reliability l D o w n o a d e d f r o m h t t p : / / d i r e c t . m i t . / / t e d u n e n a r t i c e - p d l f / / / / / 4 3 9 2 5 1 9 2 0 7 6 6 n e n _ a _ 0 0 1 4 8 p d t . f b y g u e s t t o n 0 7 S e p e m b e r 2 0 2 3 Figure 3. Number of functional connections with “good” reliability by node. On an inﬂated brain we show, for each node of the Brainnetome, Glasser, and Gordon atlases, the number of functional connections involving the node with intraclass correlation coefﬁcient = 0.60–0.75. ; Z = 152.21, p < 1 × 10−32 Effect of Global Signal Regression on Reliability GSR signiﬁcantly decreased ICC for all atlases (respectively, Z = 92.35, p < 1 × 10−32 ; Z = 188.58, p < 1 × 10−32 ). In particular, in all atlases performing GSR led the reliability of most edges to go from “fair” to “poor”. The ratio of “poor” edges increased from 29% to 53% in the Brainnetome Atlas, 31% to 61% in the Glasser, and from 47% to 69% in the Gordon. Conversely, the ratio of “fair” edges decreased from 63% to 33%, from 61% to 31% and from 47% to 24%, respectively. The ratio of “good” edges, however, marginally increased for the Brainnetome and Gordon atlases (from 8% to 12% and from 6% to 7%, respectively). Also, GSR increased the ratio of “excellent” edges in all atlases: from 0.1% to 2% in the Brainnetome, from 0.1% to 0.4% in the Glasser, and from 0.08% to 0.4% in the Network Neuroscience 933 Human functional connectome reliability l D o w n o a d e d f r o m h t t p : / / d i r e c t . m i t . t / / e d u n e n a r t i c e - p d l f / / / / / 4 3 9 2 5 1 9 2 0 7 6 6 n e n _ a _ 0 0 1 4 8 p d t . Figure 4. Connections with “excellent” reliability. On a transparent brain we show the connec- tions having “excellent” reliability (intraclass correlation coefﬁcient > 0.75) in the Brainnetome,
vidrio, and Gordon atlases. These consistently involved the superior parietal and middle temporal
lobes as well as the dorsolateral prefrontal cortex. ICC = intraclass correlation coefﬁcient.

gordon (Mesa 1 y figura 1). GSR also reduced the reliability of average FC of resting-state
redes (Z = 78, pag = 4.88 × 10−4
; Mesa 2 y figura 5).

Effect of Edge Strength on Reliability

En general, the distribution of edges in the functional connectome had its highest density in an
interval of r = 0.10–0.30 and a corresponding reliability ranging from “poor” to “fair” (ICC =
0.30–0.50) (Cifra 6).

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10−32

; rho = 0.79, pag < 1 × 10−32 Edge strength was signiﬁcantly correlated to ICC in all three atlases (rho = 0.76, p < 1 × ; rho = 0.81, p < 1 × 10−32 ). However, the FC of edges with “excellent” reliability varied across a wide range of strengths for each of the atlases: Brainnetome, r = 0.25–0.61 (median = 0.40), Glasser r = 0.12–0.61 (median = 0.34), and Gordon r = 0.13–0.44 (median = 0.22). The range was even broader for edges with “good” Network Neuroscience 934 Human functional connectome reliability Figure 5. Reliability of known resting-state networks. We show the ICC and conﬁdence inter- vals for the average connectivity within known resting-state networks deﬁned in accordance to Gordon et al. (2016). ICC = intraclass correlation coefﬁcient; No GSR = no global signal regres- sion; GSR = global signal regression; CI = conﬁdence interval; DMN = default mode network; PAO = parieto-occipital; FP = fronto-parietal; SAL = salience; COP = cingulo-opercular; MEP = medial-parietal; DAN = dorsal attention network; VAN = ventral attention network; VIS = visual; SMH = supplementary motor (hand); SMM = supplementary motor (mouth); AUD = auditory. Table 2. Reliability of known resting-state networks. ICC Upper CI Lower CI GSR− 0.6485 0.7256 0.6631 0.6252 0.6744 0.7153 0.6426 0.6648 0.6437 0.6413 0.6350 0.7095 GSR+ 0.5900 0.6783 0.5873 0.4953 0.6661 0.6960 0.5625 0.6454 0.5918 0.6050 0.5640 0.6664 GSR− 0.6862 0.7562 0.6995 0.6648 0.7098 0.7469 0.6808 0.7011 0.6818 0.6796 0.6739 0.7417 GSR+ 0.6326 0.7133 0.6301 0.5449 0.7022 0.7294 0.6072 0.6834 0.6342 0.6464 0.6086 0.7025 GSR− 0.6074 0.6918 0.6233 0.5820 0.6356 0.6804 0.6010 0.6251 0.6022 0.5996 0.5927 0.6741 GSR+ 0.5439 0.6398 0.5410 0.4423 0.6265 0.6593 0.5142 0.6040 0.5458 0.5601 0.5159 0.6269 DMN PAO FP SAL COP MEP DAN VAN VIS SMH SMM AUD Note. We show the ICC and conﬁdence intervals for the average connectiv- ity within known resting-state networks deﬁned in accordance to Gordon et al. (2016). ICC = intraclass correlation coefﬁcient; GSR− = no global signal regres- sion; GSR+ = global signal regression; CI = conﬁdence interval; DMN = default mode network; PAO = parieto-occipital; FP = fronto-parietal; SAL= salience; COP = cingulo-opercular; MEP = medial-parietal; DAN = dorsal attention net- work; VAN = ventral attention network; VIS = visual; SMH = supplementary motor (hand); SMM = supplementary motor (mouth); AUD = auditory. Network Neuroscience 935 l D o w n o a d e d f r o m h t t p : / / d i r e c t . m i t . t / / e d u n e n a r t i c e - p d l f / / / / / 4 3 9 2 5 1 9 2 0 7 6 6 n e n _ a _ 0 0 1 4 8 p d . t f b y g u e s t t o n 0 7 S e p e m b e r 2 0 2 3 Human functional connectome reliability l D o w n o a d e d f r o m h t t p : / / d i r e c t . m i t . / t / e d u n e n a r t i c e - p d l f / / / / / 4 3 9 2 5 1 9 2 0 7 6 6 n e n _ a _ 0 0 1 4 8 p d . t f b y g u e s t t o n 0 7 S e p e m b e r 2 0 2 3 Figure 6. Effects of edge strength on reliability. Plotting the strength of each edge of the Brainnetome, Glasser, and Gordon atlases versus their ICC (left) showed that edges with higher FC were mostly in the “fair” reliability range (ICC = 0.40–0.60). FC of edges with higher reliability (ICC > 0.7) were mostly between r = 0.10 and r = 0.70, and edges with very low reliability (CPI < 0.30) also had low FC (r < 0.20). The distribution of edges (right) had its highest density in an interval of r = 0.10–0.30 and a corresponding reliability ranging from “poor” to “fair” (ICC = 0.30–0.50). FC = functional connectivity; ICC = intraclass correlation coefﬁcient; r = Pearson correlation coefﬁcient. reliability (respectively, r = −0.06–0.85 (median = 0.34), r = −0.04–0.80 (median = 0.25), and r = −0.01–0.80 (median = 0.21)) as well as “fair” reliability (respectively, r = −0.13–0.83 (median = 0.20), r = −0.08–0.72 (median = 0.10), and r = −0.05–0.60 (median = 0.10)). Edges with poor reliability (ICC < 0.40) tended to have lower FC in all three atlases: res- pectively, r = −0.04–0.45, median = 0.08; r = −0.04–0.23, median = 0.04; r = −0.03–0.22, median = 0.03. Network Neuroscience 936 Human functional connectome reliability Effects of Thresholding on Reliability ; rho = −1, p = 5.98 × 10−6 In all three atlases, the median rate of consistent edges signiﬁcantly decreased as absolute thresholds became more stringent (rho = −0.97, p = 1.46 × 10−12 ; rho = −0.90, p = 1.77 × 10−7 ; rho = −0.56, p = 0.01). The same was true for relative thresholds (rho = −1, p = 5.98 × 10−6 ). The effect of absolute thresholding was a sharp decline in the median ratio of consistent edges, such that from r > 0.45 upwards, edges with “excellent” consistency ratio were only around 1% of those
retained at least once. Using a relative threshold, the median ratio of consistent edges showed
a linear decline as the thresholding became more stringent, mirrored by a proportional increase
in edges demonstrating a “poor” consistency ratio (Supporting Information Tables S1–S3 and
Cifra 7).

; rho = −1, pag = 5.98 × 10−6

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Cifra 7. Effects of thresholding on edge retention. In the Brainnetome, vidrio, and Gordon atlases, for each absolute and relative threshold
we show the proportion of edges that are consistently retained. As a measure of consistency, we use the number of participants in which the
edge was retained at both time points divided by the ones in which it was retained at least once. Por conveniencia, we then use the values
deﬁned in Cicchetti (1994) to plot the ratio of edges having poor (ratio < 0.40), fair (ratio = 0.40–0.60), good (ratio = 0.60–0.75), or excellent (ratio > 0.75) consistencia. For absolute thresholds (izquierda) all edges below the value are set to 0; for relative ones (bien) only the top percent
corresponding to the threshold is retained. r = Pearson correlation coefﬁcient.

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Cifra 8. Effects of thresholding on reliability. In the Brainnetome, vidrio, and Gordon atlases, for each absolute and relative threshold we
show the proportion of edges having poor (CPI < 0.40), fair (ICC = 0.40–0.60), good (ICC = 0.60–0.75), or excellent (ICC > 0.75) fiabilidad.
In this calculation, only subjects for which the edge was retained in both sessions were considered. For absolute thresholds (izquierda) all edges
below the value are set to 0; for relative ones (bien) only the top percent corresponding to the threshold is retained. ICC = intraclass correlation
coefﬁcient; r = Pearson correlation coefﬁcient.

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For edges retained at both time points, median ICC decreased as a result of increasingly
stringent absolute thresholds. This correlation was signiﬁcant for two out of three atlases
(rho = −0.63, pag = 0.40 × 10−2
; rho = −0.18, pag = 0.48; rho = −0.52, pag = 0.03). El
ICC of most edges that were consistently retained after absolute thresholding was “poor.” With
increasingly stringent relative thresholds, median ICC showed a slight increase in all atlases
(for the most stringent threshold: 0.06, 0.13, 0.16, respectivamente) and was signiﬁcantly corre-
lated to the threshold (rho = 0.95, p = 5.95−6
; rho = 0.99,
pag = 6.27 × 10−6
). After relative thresholding, the largest proportion of consistently retained
edges was “fairly” reliable. En particular, strict relative thresholds saw a slight increase in con-
sistently retained edges that had “excellent” reliability, mirrored by a decline in “fair” ones
(Supporting Information Table S4–S6 and Figure 8).

; rho = 0.99, pag = 6.27 × 10−6

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Human functional connectome reliability

Conﬁrmation of Results in Nonrelated Subsample

In a smaller subsample of unrelated participants, without performing GSR, the majority of
edges still had “fair” reliability (Supporting Information Table S7 and Figure S3).

When examining resting-state networks, most still had “good” reliability (Secundario

Information Table S8 and Figure S4).

The effects of GSR were similar to the ones observed in the full sample: it greatly increased
the proportion of “poor” edges, but also caused a small increase in “excellent” ones. Para
redes en estado de reposo, GSR decreased ICC of most networks (Supporting Information Tables
S7–S8 and Figure S4).

The effects of thresholding were also conﬁrmed in the unrelated subsample. We observed
a decrease of consistency of edges retained with increasingly strict thresholds (Secundario
Information Tables S9–S11 and Figure S6). The median ICC of consistently retained edges
was not strongly impacted by thresholding, but strict relative thresholds saw an increase in the
proportion of “excellent” edges and a decrease of “fair” ones (Supporting Information Tables
S12–S14 and Figure S7).

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Conﬁrmation of Results Using Different ICC Intervals

Using a different binning scheme of ICC with ﬁve instead of four classes, our results were
substantially unchanged, except for the name of the bin containing most edges (Secundario
Information Table S15 and Figure S7). According to this alternative nomenclature, most edges
had “moderate” reliability, instead of “fair” for the previous nomenclature. Edges previously
classiﬁed as having “poor” reliability were better differentiated into a larger proportion with
“fair” reliability and a minority with “slight” reliability. Edges with “substantial” and “perfect”
reliability were still a minority, and results for them were comparable to the ones for “good”
and “excellent” edges in the previous nomenclature.

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Consistently with our previous results, average network connectivity had “substantial”

fiabilidad.

The effects of GSR were similar to the ones observed with the previous bins: it greatly in-
creased the proportion of “slight” and “fair” edges, but also caused a small increase in “perfect”
unos.

The effects of thresholding were also conﬁrmed using a different binning scheme of ICC.
We observed a decrease of consistency of edges retained with increasingly strict thresholds
(Supporting Information Tables S16–S18 and Figure S8). Strict relative thresholds saw an in-
crease in the proportion of “substantial” edges and a decrease of “moderate” ones (Tables
S19–S21 and Figure S9).

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DISCUSIÓN

En este trabajo, we investigated the short-term test-retest reliability of FC in a large publicly avail-
able dataset. We conclude that a small fraction of the intrinsic connectome has good to ex-
cellent test-retest reliability. En particular, connectivity between frontal, parietal and temporal
areas appears to be especially stable over short timescales. También, while unreliable edges are
generally weak in terms of average FC, the most reliable edges are not necessarily strong.
Methodologically, we demonstrate that averaging connections within known networks and

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Human functional connectome reliability

not performing global signal regression or thresholding are practices that, en general, improve
fiabilidad.

First of all, our results provide theoretical insights about the variability in the functional
architecture of the human brain. Nosotros mostramos que, regardless of atlas, reliability of the vast ma-
jority of edges of the functional connectome is only “fair.” Still, thousands of connections had
“good” reliability, and less than a hundred were “excellent.” This is in contrast with a previous
estudiar, which reported that the “good” and “excellent” edges combined were more than 25% de
the functional connectome when considering 30 minutes of resting state (Elliott et al., 2019).
This discrepancy might be due to the much lower sample size of the previous study (norte = 33),
which might not have had sufﬁcient power to detect low ICC values. En efecto, with only two
observations per subject, Por ejemplo, al menos 152 subjects are needed to detect an ICC = 0.20
with power = 80% and alpha = 0.05 (Bujang & Baharum, 2017). En tono rimbombante, when consider-
ing the distribution of the most reliable edges in the brain, some consistent patterns emerged
across all atlases. No es sorprendente, connections involving areas prone to susceptibility artifacts,
like the subgenual anterior cingulate cortex and inferior temporal lobe, had the lowest aver-
age reliability, probably because of lower signal-to-noise ratio. En segundo lugar, in line with recent
meta-analytic evidence (Noble et al., 2019), the most reliable connections involved nodes in
the superior parietal, middle temporal lobes, and dorsolateral prefrontal cortex. This pattern
bears a striking resemblance to what has been labeled the fronto-parietal network (Marek &
Dösenbach, 2018). This network is implicated in cognitive control and interacts with other
networks implicated in this function, such as the cingulo-opercular and salience networks
(Marek et al., 2015; Power et al., 2013). It undergoes functional changes through develop-
mento, increasing in both strength and ﬂexibility from childhood to adulthood (Chai et al.,
2017). Fundamentalmente, topography of the fronto-parietal network is highly variable between indi-
viduals, which might explain the very high ICC we detected for these connections (Laumann
et al., 2015). Curiosamente, the average connectivity of the fronto-parietal network, as deﬁned
por (Gordon et al., 2016), had only “good” reliability, despite presence of several “excellent”
edges in this network as outlined above, suggesting that averaging connections might reduce
the impact of the relatively rare “excellent” ones.

Our work informs future studies wanting to focus on portions of the functional connec-
tome that combine high between-subjects reliability with low within-subjects reliability, semejante
as those investigating potential clinical biomarkers or correlations with behavioral measures
or self-reports. En particular, the fronto-parietal network has also been shown to be disrupted
across a wide range of psychiatric disorders (Cole y col., 2014). This ﬁnding, combined with
the excellent reliability of FC we have shown for this network, highlights that the functional
connections between dorsolateral prefrontal, superior parietal, and middle temporal cortices
are excellent potential targets for biomarker development in psychiatry. To aid future studies
wishing to focus on speciﬁc edges depending on their reliability, we provide connectivity
“masks” for each atlas in our GitHub repository, eso es, connectivity matrices in which each
edge is labeled with its reliability (poor, fair, bien, or high), connectivity matrices with the
ICC values for each edge, and connectivity matrices with the upper and lower conﬁdence in-
tervals for our ICC estimates (“ICC_matrices/”). Our results also indicate that another viable
complimentary approach for biomarker studies is computing average connectivity of known
resting-state networks by using an atlas, which consistently returns values that have “good”
fiabilidad. This is in line with previous studies which showed, Por ejemplo, that average con-
nectivity of the default mode network is a good candidate measure for clinical translation and
that connectivity within established networks is reliable in general (Baile et al., 2017; Zuo &

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Human functional connectome reliability

Xing, 2014). This also offers a very strong reduction of the number of features (from tens of
thousands to a dozen), which might be desirable, depending on the study aims.

From a methodological point of view, we were able to test the consequences on reliability
of atlas choice, GSR, and thresholding. Concerning the choice of atlas, both the Brainnetome
and Glasser atlases had comparable proportion of “good” and “excellent” edges, mientras que el
Gordon Atlas showed consistently lower reliability. The Glasser Atlas also had the highest
absolute number of connections, which led to identiﬁcation of the highest number of “excel-
lent” edges. Researchers wishing a higher number of reliable edges could consider this atlas,
remembering, sin embargo, that this comes at the cost of more “poor” and “fair” edges as well.
GSR consistently led the majority of edges having “fair” reliability to having “poor” reliability.
También, it consistently reduced ICC of average FC within resting-state networks. This is probably
due to the fact that regressing the average signal from each individual subject eliminates the
between-subject variability due to differences in the signal baseline, similarly to what has been
shown for contrasts between conditions in task fMRI data (Haller et al., 2018). Curiosamente,
the ratio of “excellent” edges consistently increased, suggesting that some edges might beneﬁt
from GSR. Por lo tanto, as previous studies suggested, applying GSR or not can reveal com-
plimentary insights and the choice should be carefully made depending on the study aims
(Murphy & Fox, 2017).

Examining the role between FC strength, averaged across sessions, and reliability unveiled
a complex relationship. While unreliable edges are generally weak in terms of FC, the oppo-
site is not true: the most reliable edges are not necessarily strong and lie in a broad interval of
r values (r = 0.12–0.61 depending on atlas). Por lo tanto, thresholding the functional connec-
tome based on absolute or relative edge strength had, en general, detrimental consequences
on reliability. First of all, applying an increasingly stringent threshold for the two sessions
from each subject independently led to an increasingly large amount of connections not being
consistently retained. Por ejemplo, when retaining only connections with r > 0.2 or higher,
most of the connections that survived thresholding did so in one session but not in the other.
The impact of relative thresholding was overall less dramatic compared to absolute thresh-
olding, with the median proportion of reliably kept edges crossing 0.50 only for a threshold
of ∼0.50. Examining the impact on ICC of the edges consistently retained after thresholding
showed only a slight improvement on the median ICC and ratio of “excellent” edges for in-
creasingly stringent relative thresholds. También, we calculated ICC only for consistently retained
bordes, which we showed to be relatively rare. En general, our results suggest that the practice of
thresholding functional connectomes based on edge strength should be avoided in biomarker
and correlational studies, especially in the case of absolute thresholds.

Our study is not without limitations. First of all, given that a signiﬁcant portion of variance
in FC is explained by genetics (Adhikari et al., 2018; Elliott et al., 2019; Reineberg et al., 2018),
our analyses on the complete Healthy Young Adult dataset might have suffered from reduced
between-subjects variability and our results might have overall underestimated reliability. A
recent study on the same dataset (Reineberg et al., 2018) concluded that genetic inﬂuence
on FC is only moderate. In any case, performing the same analyses on the subset of unrelated
participants returned results that were overall comparable. Still, the ICC estimates on unrelated
participants had broader conﬁdence intervals compared to those of our main analysis because
of the lower number of participants. To be certain of removing the effect of family on reliability,
a target for future studies is the analysis of very large cohorts of mostly unrelated subjects.
También, the inevitable uncertainty of our ICC estimates should be considered. To allow this, nosotros
provide on GitHub for each atlas edge-wise ICC values and their upper and inferior conﬁdence

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Human functional connectome reliability

interval bounds. En general, our large sample size made the width of these conﬁdence intervals
relatively small and they did not cross the boundary between reliability bins for most edges.
Still, we highlight that assignment to a bin of edges with an ICC close to its threshold value is
uncertain. In the present work, we also used an ANOVA-derived ICC, which resulted in a very
small fraction of results having negative values. Linear-mixed effect implementations of ICC
have been recently proposed for functional neuroimaging (Chen et al., 2018). The underlying
model being identical, these would have produced identical results on our data, except that
the negative values would have been zero. Since our main ﬁndings are based on reliability
bins and rank statistics, they would be unchanged with these methods. Another limitation is
that we assessed reliability over a very short time span. While this allowed us to use a very
large sample, our results might not be comparable to the ones from longitudinal designs with
repeated measures across weeks or months. Finalmente, we plan on expanding our analyses to
additional parcellations in the future and provide additional connectivity “masks” for atlases
that are widely used by the neuroimaging community, such as the Yeo and Desikan-Killiany
(Desikan et al., 2006; Thomas Yeo et al., 2011).

CONCLUSIONS

En conclusión, our ﬁndings advance the efforts to identify reliable measures for the ﬁeld of
functional neuroimaging. We demonstrate that a readily identiﬁable, albeit small, portion of
the intrinsic functional connectome is characterized by good to excellent reliability over time.
Fronto-temporo-parietal connections appear to be especially reliable over short timescales,
and thus suited to delineation of trait-like markers of both normative and disrupted cogni-
tive functions. Averaging of connections within established circuits without thresholding also
yields a stable FC metric. Harnessing this knowledge offers one way forward for the study of in-
dividual trait-like properties of the human connectome or for disciplines focused on disorders
of functional brain connectivity, such as Psychiatry.

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SUPPORTING INFORMATION

Supporting Information for this article is available at https://doi.org/10.1162/netn_a_00148.
All data used in this study is in the public domain and was downloaded from https://db.
humanconnectome.org/. All code used for the analyses is in the public domain and avail-
able at https://github.com/leotozzi88/reliability_study.

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CONTRIBUCIONES DE AUTOR

Leonardo Tozzi: Conceptualización; Curación de datos; Análisis formal; Writing – Original Draft;
Writing – Revisar & Editing. Scott L. Fleming: Conceptualización; Writing – Revisar & Edit-
En g. Zachary D. taylor: Conceptualización; Writing – Revisar & Editing. Cooper D. Raterink:
Conceptualización; Writing – Revisar & Editing. Leanne Williams: Conceptualización; Fondos
adquisición; Administración de proyecto; Recursos; Supervisión; Writing – Original Draft; Writing –
Revisar & Editing.

INFORMACIÓN DE FINANCIACIÓN

Leanne Williams, NIMH, Award ID: U01MH109985.

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