RECHERCHE
Implanting intracranial electrodes does not affect
spikes or network connectivity in nearby or
connected brain regions
Erin C. Conrad1, Russell T. Shinohara2,3,4, James J. Gugger1, Andrew Y. Revell5, Sandhitsu Das1,
Joel M. Stein6, Eric D. Marsh1,7, Kathryn A. Davis1*, and Brian Litt1*
1Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvanie, Etats-Unis
2Department of Biostatistics, Epidemiology, and Informatics, University of Pennsylvania, Philadelphia, Pennsylvanie, Etats-Unis
3Penn Statistics in Imaging and Visualization Center, University of Pennsylvania, Philadelphia, Pennsylvanie, Etats-Unis
4Center for Biomedical Image Computing and Analytics, University of Pennsylvania, Philadelphia, Pennsylvanie, Etats-Unis
5Medical Scientist Training Program, University of Pennsylvania, Philadelphia, Pennsylvanie, Etats-Unis
6Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvanie, Etats-Unis
7Division of Child Neurology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvanie, Etats-Unis
*These authors contributed equally.
Mots clés: EEG intracrânien, Drug-resistant epilepsy, Electrode implantation, Interictal spikes,
Functional networks
ABSTRAIT
To determine the effect of implanting electrodes on electrographic features of nearby and
connected brain regions in patients with drug-resistant epilepsy, we analyzed intracranial EEG
recordings from 10 patients with drug-resistant epilepsy who underwent implant revision
(placement of additional electrodes) during their hospitalization. We performed automated
spike detection and measured EEG functional networks. We analyzed the original electrodes
that remained in place throughout the full EEG recording, and we measured the change in
spike rates and network connectivity in these original electrodes in response to implanting
new electrodes. There was no change in overall spike rate pre- to post-implant revision (t (9) =
0.1, p = 0.95). The peri-revision change in the distribution of spike rate and connectivity across
electrodes was no greater than chance (Monte Carlo method, spikes: p = 0.40, connectivité:
p = 0.42). Electrodes closer to or more functionally connected to the revision site had no greater
change in spike rate or connectivity than more distant or less connected electrodes. Changes
in electrographic features surrounding electrode implantation are no greater than baseline
fluctuations occurring throughout the intracranial recording. These findings argue against an
implant effect on spikes or network connectivity in nearby or connected brain regions.
RÉSUMÉ DE L'AUTEUR
We tested whether implanting intracranial electrodes affected interictal spike rates or
functional connectivity on preexisting electrodes. We found that the change in electrographic
features following electrode implantation was no larger than the baseline fluctuations
occurring throughout the intracranial recording. Our results argue against an implant effect
on spikes or network connectivity in nearby or connected brain regions.
un accès ouvert
journal
Citation: Conrad, E. C., Shinohara,
R.. T., Gugger, J.. J., Revell, UN. Y., Le,
S., Stein, J.. M., Marais, E. D., Davis,
K. UN., & Un peu, B. (2022). Implanting
intracranial electrodes does not affect
spikes or network connectivity in
nearby or connected brain regions.
Neurosciences en réseau, 6(3), 834–849.
https://doi.org/10.1162/netn_a_00248
EST CE QUE JE:
https://doi.org/10.1162/netn_a_00248
Informations complémentaires:
https://doi.org/10.1162/netn_a_00248;
https://github.com/erinconrad
/interictal_hubs/tree/main
/implant_analyses
Reçu: 8 Décembre 2021
Accepté: 1 Avril 2022
Auteur correspondant:
Erin C. Conrad
erin.conrad2@pennmedicine
.upenn.edu
Éditeur de manipulation:
Sarah Muldoon
droits d'auteur: © 2022
Massachusetts Institute of Technology
Publié sous Creative Commons
Attribution 4.0 International
(CC PAR 4.0) Licence
La presse du MIT
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Testing the implant effect
INTRODUCTION
Patients with drug-resistant epilepsy (DRE) may benefit from surgery or neurostimulation
(French, 2007; Kwan et al., 2011; Wiebe et al., 1999). EEG intracrânien (IEEG) monitoring is
used to guide surgical planning in these patients. This process involves implanting intracranial
electrodes and then recording EEG for days to weeks in order to evaluate the electroclinical
features of seizures as well as interictal activity. Studies in humans and animals show that
implanting electrodes creates histological changes that may affect spike rates and other brain
network features (Liu et al., 1999; Morrell, 2011; Polikov et al., 2005; D. UN. Sun et al., 2008;
F. T. Sun et al., 2018; Ung et al., 2017; Van Kuyck et al., 2007). Clinical reports raise the con-
cern that this “implant effect” may begin immediately and may affect our ability to localize
seizure generators using intracranial recordings (Fountas et al., 2004; Katariwala et al., 2001;
Kovac et al., 2014; Roth et al., 2012; Schulze-Bonhage et al., 2010). Our understanding of the
effect of electrode implantation on EEG features is limited by the fact that the agents used to
study the effect—intracranial electrodes—are the same agents thought to cause it. In other
words, we cannot measure the pre-implant IEEG baseline.
One risk of intracranial recording is undersampling important brain regions. To correct this,
a minority of patients undergo implant revision during their hospitalization, in which addi-
tional electrodes are implanted to address hypotheses that emerge from initial implant record-
ings (Lee et al., 2014). These cases provide the unique opportunity to observe the IEEG before
and after additional electrodes are implanted. Here we analyze data from 10 patients with DRE
who underwent implant revision during the course of presurgical IEEG recording. We measure
interictal spikes and functional connectivity. We measure the change in EEG features from pre-
to post-implant revision on the original electrodes present throughout the entire recording.
Suivant, to test the hypothesis that electrode implantation affects nearby or connected brain
régions, we determine whether there is a greater pre- to post-implant change in EEG features
on electrodes closer to or more functionally connected to the site of implant revision.
RÉSULTATS
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Of the original 16 patients studied, six patients were excluded at the spike validation step (five
for poor detector accuracy, and one for sparse spikes). Patients had heterogeneous implant revi-
sion targets, anti-seizure medication (ASM) changes, seizure localizations, and other clinical
data (Tableau 1). The average duration of total EEG recording was 16.6 jours (range 13–26). Un
average of 165,061 spikes were detected (range 33,366–364,955; Table S1 in the Supporting
Information). Across patients, the original electrode closest to the revision site was on average
7.4 (SD 6.4) mm from the revision site.
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Different Measures of Proximity to the Revision Site Agree With Each Other
We examined the correlation between three variables representing different measures of prox-
imity to revised electrodes. For each original electrode we measured (un) the distance to its
nearest revised electrode, (b) the co-spike index (the percentage of spikes on the original elec-
trode that co-occur in close temporal proximity with one of the revised electrodes), et (c) le
functional connectivity (the average EEG Pearson correlation coefficient with the revised elec-
trodes). Across patients, there was a negative correlation between distance and co-spike index
(mean r = −0.52, t test of individual patient Fisher’s r- to z-transformed correlation coefficients:
t(9) = −8.70, p < 0.001). There was also a negative correlation between distance and func-
tional connectivity (mean r = −0.37, t(9) = −4.78, p = 0.001), and a positive correlation
between co-spike index and functional connectivity (mean r = 0.42, t(9) = 3.50, p = 0.007)
Co-spike index:
The proportion of spikes in a given
original electrode that co-occurred
within 50 ms on any revised
electrode. The tendency for original
electrodes to have spikes occur
simultaneously with those on revised
electrodes.
Network Neuroscience
835
t
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ID Sex Age
F
1
Revision target
20 Mesial temporal,
peri-lesional
Table 1.
Clinical information.
Peri-revision
ASM changes
Full restart
Seizure localization
Peri-lesional w/
temporal spread
Seizure onset in
added electrodes
Yes
Surgery
ATL
2-year
ILAE
Class 2
Complications
Subdural hematoma
with first implant
42 Multiple frontal
No change
Unclear
No
None
N/A
None
F
F
F
F
F
2
3
4
5
6
7
8
9
10
targets, occipital
(continued
home ASMs)
40
Parietal
Partial restart
Parietal w/
61
Parietal, temporal
neocortical
Paused wean
temporal spread
Temporal neocortical
w/ diffuse spread
46
Inferior frontal,
Full restart
Perisylvian eloquent
temporal neocortical
cortex
57
Amygdala, cingulate,
insula, orbitofrontal
Full restart
Mesial temporal
and orbitofrontal
M
33
Peri-lesional
Partial restart
Diffuse hemispheric
M
42
Peri-lesional
No change
Mesial temporal
(off all ASMs)
and peri-lesional
F
F
37
Temporal neocortical
Partial restart
Mesial temporal
40
Peri-lesional
Continued
wean
Unknown
Yes
Yes
Yes
Yes
No
Yes
No
No
ATL
Class 1
RNS
Class 4
Temporal
ablation
RNS
DBS
Class 5
Class 4
Recent
surgery
None
N/A
Temporal
ablation
Recent
surgery
DBS
Recent
surgery
None
None
None
None
None
None
None
None
Abbreviations: F = female, M = male. ASM = anti-seizure medication. ATL = anterior temporal lobectomy, RNS = responsive neurostimulation, DBS = deep brain stimulation. ILAE =
International League Against Epilepsy.
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Testing the implant effect
(Figure 1). This implies that original electrodes closer to the site of implant revision are more
connected to the new electrodes as measured by Pearson correlation and co-spiking. This
finding also provides additional validation of the automated spike detections and functional
connectivity measurements.
There Is No Consistent Change in Spike Rate Within the Implantation
We compared spike rates between the early and late periods of both the first and second
implant (Figure 2). There was no difference in spike rates between the early and late stage
of the original electrodes in the first implant (Early M = 1.0 spikes/electrode/min, SD = 1.1;
Late M = 1.0 (0.9), t(9) = 0.1, p = 0.90), of the original electrodes in the second implant (Early
M = 1.0, SD = 1.0; Late M = 0.6 (0.4), t(9) = 1.0, p = 0.32), or of the added electrodes in the
second implant (Early M = 0.5, SD = 0.6; Late M = 0.3 (0.2), t(9) = 1.3, p = 0.23). There was
also no difference in the early to late relative spike rate change between the original electrodes
in the first versus the second implant (first relative change M = 0.6, SD = 1.3; second relative
change M = 0.5, SD = 1.7; t(9) = 0.04, p = 0.97) or between the original electrodes and the
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Figure 1. Study approach and concordance between different measures of proximity to the revision site. (A) Conceptual diagram demon-
strating reported effects of implanting intracranial electrodes, including local injury, hemorrhage, and non-habitual seizures. (B) Conceptual
diagram describing our study’s approach. We examined patients who underwent intracranial implantation and subsequently had new elec-
trodes placed. We examined the relative change in the electrographic features on the old electrodes in response to implanting new electrodes.
(C–H) Concordance between measures of proximity to the revision site. C–E show results for a single patient (Patient 1) as an example. F–H
show aggregate results across patients. (C, F) The correlation between distance from the nearest added electrode and the co-spike index. (D, G)
The correlation between distance from the nearest added electrode and functional connectivity to the added electrodes. (E, H) The correlation
between functional connectivity to the added electrodes and co-spike index. For subfigures C–E, each circle represents the values for a single
original electrode in the example patient. For subfigures F–H, each circle is the Pearson correlation coefficient for a single patient, and error
bars represent the 95% confidence intervals on the correlation coefficient (obtained by bootstrapping with 10,000 iterations). The p value is
that from a two-sided t test testing whether the Fisher r- to z-transformed correlation coefficients across patients were significantly different
from 0.
Network Neuroscience
837
Testing the implant effect
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Figure 2. Spike rates across time within the implant. (A) Raster plot showing the spike rate across original electrodes at different times for an
example patient (Patient 5). Gray periods are periods of automatically detected disconnection or severe electrode artifact. The vertical maroon
dotted line shows the time of the implant revision. The precise time of the implant revision during the period of disconnection is unknown.
Horizontal bars above the graph show the time periods defined as Early Implant 1, Late Implant 1, Early Implant 2, and Late Implant 2. (B) The
change in spike rate from early in the implant to late in the implant for both implants. Each line represents data for a single patient. The line
connects the average spike rate in the early period to that in the late period. The leftmost set of lines represents the original electrodes in the
first implantation. The middle lines represent the original electrodes in the second implantation. The rightmost lines represent the added elec-
trodes in the second implantation. There was no consistent change in spike rates between the early and late period for any set of electrodes.
Also, there was no difference in the relative early to late spike rate change between the first and second implantation, or between the original
and added electrodes in the second implantation. (C) The correlation between the early to late period time difference and the relative spike rate
change. The leftmost plot shows data for Implant 1, the middle plot shows the original electrodes from Implant 2, and the rightmost plot shows
the added electrodes from Implant 2. There was no correlation between the early to late time difference and the relative spike rate change for
either implant.
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added electrodes in the second implant period (original electrodes relative change M = 0.5,
SD = 1.7; added electrodes relative change M = −0.0, SD = 0.8; t(9) = −1.0, p = 0.33). This
was also true for each tested duration ranging from 3 to 60 hr defining the early and late
periods (Table S2 in the Supporting Information). There was no correlation between the early
to late duration and the relative spike rate change for the first implant (ρ = 0.05, p = 0.89), the
Network Neuroscience
838
Testing the implant effect
Spike stability:
Spearman rank correlation between
the pre- and post-revision spike rate
distribution. The agreement between
the distribution of spike rates across
electrodes pre- to post-revision
(higher spike stability → less peri-
revision change).
Spike rate distribution:
Vector containing the average spike
rate of each electrode. The
distribution of spike rates across
electrodes.
Node strength stability:
Spearman rank correlation between
the pre- and post-revision
connectivity distribution. The
agreement between the distribution
of connectivity across electrodes pre-
to post-revision (higher node strength
stability → less peri-revision change).
Node strength:
Sum of the Pearson correlation
coefficients between the EEG signal
on a given electrode and the EEG
signals on all other electrodes. A
measure of overall connection
strength for a given electrode (higher
node strength → more connected).
Network Neuroscience
original electrodes in the second implant (ρ = −0.21, p = 0.56), or the added electrodes in the
second implant (ρ = 0.07, p = 0.86). These results imply that (a) there is no consistent change
in spike rates within either implantation, (b) the post-implantation change in spike rates is sim-
ilar between the two implants, and (c) the post-revision change in spike rates is similar
between newly added electrodes and those electrodes already in place.
Overall Spike Rate Does Not Change Surrounding Electrode Implantation
We next compared the overall spike rate between the pre- and post-revision period. No indi-
vidual patient had a larger peri-revision change in overall spike rates than expected at ran-
domly chosen time periods (Monte Carlo test with Bonferroni correction). Patient 3, who
was the only patient in whom a grid electrode configuration was added, had the largest
peri-revision spike rate change (1.1 → 3.5 spikes/electrode/min), although the Monte Carlo
analysis was not significant after Bonferroni correction (p = 0.02, α = 0.005). There was no
consistent difference across patients between the pre-revision (M = 1.0, SD = 0.9
spikes/electrode/min) and post-revision (M = 1.0, SD = 1.0 spikes/electrode/min) spike rate
(t(9) = 0.1, p = 0.95) (Figure 3B). There was also no consistent peri-revision change in spike
rate seen using any other peri-revision duration ranging from 6 to 120 hours (Table S3 in the
Supporting Information, Bonferroni correction).
The Pre- to Post-Implant Change in the Distribution of Electrographic Features Across Electrodes Is No
Larger Than Chance
We measured the peri-revision spike stability, defined as the Spearman rank correlation
between the pre- and post-revision spike rate distribution across electrodes (Figures 3C–3F).
The spike stabilities for all individual patients and aggregated across patients (M = 0.76, SD =
0.19) were no different from chance (Monte Carlo with Fisher’s method: p = 0.40). The node
strength stability (M = 0.75, SD = 0.21) was also no different from chance ( p = 0.42). The same
findings were observed examining other peri-revision interval durations (Table S3 in the
Supporting Information, Bonferroni correction). Taken together, these results imply that the dis-
tributions of spike rates and node strength across electrodes do not change more across the peri-
revision period than at other time points in the recording.
There Is No Correlation Between the Peri-Revision Change in Electrographic Features and the Proximity
to the Revision Site
We next measured the correlation between the relative change in spike rate and distance from
the site of implant revision. Figure S1 in the Supporting Information shows the individual
patient correlations. Although several individual patients had significant correlations (with
inconsistent directions), no correlation was greater than that observed at randomly chosen
pseudo-revision times (Monte Carlo test with Bonferroni correction). This suggests that the cor-
relations on individual patient levels are incidental, resulting from the high spatial autocorre-
lation in spike rates (electrodes close to each other demonstrate similar changes in spike rates)
and are not specific to the timing of the implant revision itself. The average correlation across
patients between relative spike rate change and distance from the revision site was ρ = −0.15,
which was not significant (t (9) = −1.6, p = 0.14). There was no significant correlation seen for
other choices of peri-revision durations (Figures 4A and 4C; Table S3 in the Supporting Infor-
mation). There was also no consistent correlation between relative spike rate change and func-
tional connectivity with the revision site (average ρ = 0.09, t (9) = 1.2, p = 0.26) or between
relative spike rate change and co-spike index with the revision site (average ρ = 0.08, t (9) =
0.8, p = 0.46).
839
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Testing the implant effect
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Figure 3. Change in electrographic features from pre- to post-implant revision. (A) The overall spike rate for an example patient (Patient 4).
The x-axis shows the time in hours, and the y-axis shows the spike rate across time. The maroon dotted vertical line shows the time of the
implant revision. Green and purple lines denote, respectively, the times defined as the pre-revision and post-revision periods assuming a 24-hr
peri-revision interval duration. (B) The average spike rate in the pre- and post-revision periods for each patient. Each line represents one patient
and connects the average spike rate in the pre-revision period to that in the post-revision period. There was no significant group change in
spike rates surrounding implant revision (paired t test, α = 0.05). (C) Spike stability in the 24-hr peri-revision interval. Each circle in the “true”
column shows the spike stability, defined as the correlation in spike rate distribution across electrodes between the pre- and post-revision time
periods. Each circle in the “Monte Carlo” column shows the same statistic, but averaged across randomly chosen pseudo-revision times. (D)
Node strength stability in the 24-hr peri-revision interval. Each circle in the “true” column shows the correlation in node strength distribution
across electrodes between the pre- and post-revision time periods. Each circle in the “Monte Carlo” column shows the same statistic, but
averaged across randomly chosen pseudo-revision times. (E and F) The spike and node strength stability, respectively, when comparing
pre- and post-revision periods for different peri-revision interval durations. Both the true and Monte Carlo statistics are shown. Error bars show
standard deviations across patients and, in the case of the Monte Carlo statistics, Monte Carlo iterations. ns = not significant.
We also measured the correlation between the relative change in node strength and dis-
tance from the revision site. The average correlation between relative node strength change
and distance from the revision site was ρ = −0.01, which was not significant (t(9) = −0.1, p =
0.92). This result was consistent when examining different peri-revision durations (Figures 4B
and 4D; Table S3 in the Supporting Information). There was also no correlation between rel-
ative node strength change and functional connectivity with the revision site (average ρ =
0.04, t(9) = 0.5, p = 0.62) or between relative node strength change and co-spike index with
the revision site (average ρ = −0.06, t(9) = −0.5, p = 0.62). Together, these results indicate that
electrodes more proximate to the revision site (as measured by distance, EEG Pearson connec-
tivity, or co-spiking) do not experience a larger change in electrographic features surrounding
the implant revision.
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Figure 4. The effect of proximity to the revision site and anatomy on peri-revision spike and connectivity changes. (A) The correlation
between peri-revision relative spike rate change and distance from the revision site for both the true revision time (blue) and the average
and standard deviation of 1,000 randomly chosen pseudo-revision times (Monte Carlo, red; error bars indicate standard deviation across
pseudo-revision times), for a peri-revision interval duration of 24 hr. Random jitter has been added to the x-axis for visualization. (B) The
correlation between peri-revision relative node strength change and distance from the revision site for the true revision time and the average
of randomly chosen pseudo-revision times, again for a peri-revision interval duration of 24 hr. (C) The true (blue) and Monte Carlo (red) mean
and standard deviation (error bars) correlation between the relative spike rate change and distance from the revision site. The mean and
standard deviation are performed across patients and, in the case of the Monte Carlo statistics, across Monte Carlo iterations. Results for each
peri-revision duration are shown. (D) The true (blue) and Monte Carlo (red) mean and standard deviation (error bars) correlation between the
relative node strength change and distance from the revision site. Results for each peri-revision duration are shown. (E) The mean (circle) and
standard deviation (error bars) across patients of the relative change in spike rate peri-revision according to anatomical location. (F) The rel-
ative change in node strength according to anatomical location. ns = not significant.
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Peri-Revision Change in Spike Rate and Node Strength Did Not Differ Across Anatomical Locations
Across all patients, 194 electrodes (19.8%) were outside cerebral tissue; we excluded these
from analysis. Three hundred forty-nine (35.6%) of electrode contacts were in white matter,
54 (5.5%) were in mesial temporal regions, 158 (16.1%) were in temporal neocortical regions,
and 226 (23.0%) were in other locations. The mean (SD) relative spike rate change in the 24-hr
peri-implant interval was 0.3 (1.0) for white matter, 0.5 (1.4) for mesial temporal, 0.3 (0.8) for
temporal neocortical, and 0.4 (1.3) for other regions. The difference between groups was not
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Testing the implant effect
significant (Skillings-Mack χ2(3) = 0.4, p = 0.95). The mean (SD) relative node strength change
in the 24-hr peri-implant interval was −0.3 (1.3) for white matter, −0.6 (2.5) for mesial tempo-
ral, −1.5 (3.7) for temporal neocortical, and 0.3 (1.5) for other regions. The difference between
groups was not significant (Skillings-Mack χ2(3) = 2.3, p = 0.52). There was also no difference
seen for any other peri-revision duration (Table S3 in the Supporting Information).
Peri-Revision Change in Spike Rate and Node Strength Did Not Depend on Type of Added Electrode
We tested the effect of added electrode type on relative spike rate and node strength change
using a single patient (Patient 2) who had a combination of depth and subdural electrodes added.
The median relative spike rate change for original contacts within 20 mm of the nearest
added depth contacts was −0.56, which was lower than that observed for contacts within
20 mm of the closest added subdural contacts (9.80) (Mann-Whitney U test: U(Ndepth proximate =
5, Nsubdural proximate = 17) = 16.0, p = 0.041). The median relative node strength change for
original contacts within 20 mm of the closest added depth contacts was −1.17, which was not
significantly different from that for contacts within 20 mm of the closest added subdural con-
tacts (−0.77) (Mann-Whitney U test: U(Ndepth proximate = 5, Nsubdural proximate = 19) = 41.5, p =
0.70). There was no significant difference in relative feature change between electrodes close
to added depth electrodes and those close to added subdural electrodes for any other peri-
revision duration or for using a distance threshold of 10 or 30 mm (Table S4 in the Supporting
Information). Taken together, these results suggest that the implant effect does not clearly differ
by electrode type. However, the fact that this was a single-patient analysis limits interpretation.
DISCUSSION
Establishing the existence of an “implant effect” on electrographic features is challenging
without a pre-implantation baseline. In our study, we found no evidence that implanting elec-
trodes affects either the spike rate or network connectivity of preexisting electrodes.
The Change in Spike Rates Within Each Implantation Is Inconsistent Across Patients
We observed no consistent change in spike rates from early to late in the implant. Furthermore,
the relative change in spike rate within the revised implant did not differ between the original
and the newly added electrodes. This latter result argues against an implant effect producing
immediate and transient changes in spike rates. We would expect such an effect to produce a
larger change in spike rates on the newly added electrodes compared with the original elec-
trodes (which had been in place for several days by the time of the implant revision). However,
this analysis is limited by the several confounders that influence spike rates, including peri-
implant sedation, medication changes, and seizures (Spencer et al., 2008).
The Change in Electrographic Features Pre- to Post-Electrode Revision Is No Larger Than Chance
We found no consistent pre- to post-implant revision change in overall spike rates. Also, the
pre- to post-revision change in the distribution of spikes rates and network connectivity across
electrodes was no higher than that observed at random periods throughout the intracranial
recording. This suggests that the effect of implanting new electrodes on electrographic features
from other brain regions is no larger than the baseline fluctuations in these features. Some of
these fluctuations were large (Figure 3), implying substantial variability in the distribution of
spikes across electrodes throughout the recording. This highlights the importance of analyzing
prolonged durations to capture the variability in spike location when using interictal data for
surgical planning (Conrad et al., 2020; Diamond et al., 2019; Janca et al., 2018).
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The peri-revision change in spike rate and node strength did not depend on the distance to,
functional connectivity to, or co-spiking with the implant revision site. This analysis tested the
hypothesis that implanting electrodes would disproportionately affect spiking and network
connectivity in nearby or connected regions. The negative result provides further evidence
against a direct effect of electrode implantation on spike rates and network connectivity.
Peri-Revision Electrographic Changes Do Not Differ Across Anatomical Locations
We found no difference in spike rate or node strength change across anatomical locations. This
analysis tested the hypothesis that a global implant-related cause—such as anesthesia—may
have a disproportionate effect on EEG features in certain anatomical regions. Our negative
finding leaves open the possibility that a global implant-related effect causes diffuse EEG
changes. However, our other finding of no overall peri-revision spike rate change argues
against this possibility.
Limitations
We cannot exclude an exquisitely focal implant effect, influencing only the brain regions
immediately underlying the implanted electrodes. Our finding that the electrodes added in
the implant revision do not experience a larger change in spike rate compared with the orig-
inal electrodes argues against this. Future studies incorporating microelectrode recordings
could potentially probe the hyper-local effect of implanting additional macroelectrodes.
Two additional major limitations relate to the timing of electrographic changes. First, the
24-hr pause in EEG recording surrounding implant revision precluded us from studying very
brief post-implant changes. A future study incorporating intraoperative recordings from
patients undergoing implant revision could probe this effect. Finding no implant effect in
the immediate post-implantation period would support the feasibility of using immediate post-
operative data to localize seizure generators. Second, we could not study electrographic
changes that begin after recording finishes. Other studies in long-term implantations described
chronic changes in spike rates in response to implanting neurostimulation devices (F. T. Sun
et al., 2018; Ung et al., 2017).
Most patients in our study had only stereo-EEG depth electrodes added. We might expect a
larger implant effect from the implantation of grid and strip subdural electrodes, particularly in
the setting of complications such as hemorrhage and infection. In our secondary analysis of a
single patient who underwent addition of both depth and subdural electrodes, we found no
consistent difference between electrographic feature change between the original electrodes
closest to added depth electrodes and those closest to added subdural electrodes. On the other
hand, we observed a nonsignificant (p = 0.02, α = 0.005) trend of peri-revision increased
spiking in the single patient who underwent implantation of a subdural grid. This latter result
suggests that implantation of subdural grid electrodes may increase spike rates, although this
interpretation is limited by our single patient analysis.
Clinical and Research Implications
There is concern that the early post-implant period may be nonideal for gathering electrocli-
nical data to use in surgical planning. Competing evidence supports that this period may either
be pro- or anti-convulsive. In favor of the pro-convulsive hypothesis, multiple case series
reported non-habitual seizures in the first few days after implanting intracranial electrodes
(Fountas et al., 2004; Kovac et al., 2014). Important caveats to these clinical reports are
the following: (a) They represent grid/strip implantations, which have higher rates of
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pro-convulsive complications compared with depth-only implantations. (b) Some non-
habitual seizures may simply be clinically subtle scalp-negative seizures. And (c) non-habitual
seizures may result from a combination of medication wean and peri-operative sedation,
rather than from electrode implantation itself. On the other hand, other groups have reported
cases in which electrode implantation alone apparently cured the patients’ epilepsy (Katariwala
et al., 2001; Roth et al., 2012; Schulze-Bonhage et al., 2010). A similar mechanism could
plausibly cause a temporary decrease in seizures and cortical irritability. Insofar as interictal
spikes may serve as a marker for irritable, seizure-prone cortex (Goncharova et al., 2016;
Karoly et al., 2016), then these competing hypotheses could predict either an increase or a
decrease in spikes after implantation.
We found no electrographic evidence supporting either a pro- or anti-irritative implant
effect on nearby or connected brain regions. With our study’s limitations noted above, our
findings suggest that early periods (24+ hr after electrode implantation) in intracranial record-
ing represent the patient’s typical interictal network. These findings argue against discounting
early post-implantation IEEG recording for clinical use in surgical planning or in research
aimed at understanding interictal networks.
MATERIALS AND METHODS
Patient Selection, Clinical Data Review, and Intracranial Recording
This retrospective study was approved by the Hospital of the University of Pennsylvania (HUP)
Institutional Review Board. Informed written consent was obtained from each participant. We
examined 16 sequential patients with DRE who underwent IEEG recording during presurgical
evaluation at HUP and who had electrode revision during their hospitalization. Implant revi-
sion was performed to sample brain regions not captured in the original recording and hypoth-
esized to be involved in the seizure network. A board-certified epileptologist reviewed clinical
charts for clinical information and two-year International League Against Epilepsy (ILAE) sur-
gical outcomes, when available (Wieser et al., 2001). The methods for intracranial EEG record-
ing, electrode localization, and seizure identification are described in the Supporting
Information.
EEG Preprocessing, Artifact Rejection, Spike Detection, and Functional Network Calculation
EEG recording was usually paused or leads were disconnected surrounding the implant revi-
sion (mean [M] total gap in data 25.9 hr, standard deviation [SD] 10.5 hr). Five-minute con-
tinuous segments of IEEG were selected at random from every 30 min of the full recording
duration, excluding this gap and time periods containing seizures. This downsampling was
performed to reduce computation time. The EEG signal on each 5-min segment and each elec-
trode was then subjected to automated artifact detection to identify and remove periods of
heavy noise or disconnection (see the Supporting Information).
We performed automated interictal spike detection using a previously validated detector,
described in the Supporting Information (Brown et al., 2007). We next calculated the co-spike
index for each original electrode, defined as the proportion of spikes that co-occurred within
50 ms on any revised electrode (50 ms chosen based on previous studies of spike propagation;
Baumgartner et al., 1995; Bourien et al., 2005; Tomlinson et al., 2016). The co-spike index
was averaged across all post-revision EEG segments. The co-spike index measured the degree
of connectivity to the revised electrodes as measured by spiking, where a higher co-spike
index implied higher spike-related connectivity. We also calculated functional networks for
each segment by measuring the Pearson correlation coefficient between the EEG signals on
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every pair of original electrodes (Supporting Information). As a measure of functional connec-
tivity to the revised electrodes, we then calculated, for each original electrode, the Pearson
correlation coefficient with every revised electrode, averaged over all revised electrodes
and across all post-revision EEG segments.
Within-Implant Electrographic Changes
We defined an early period and a late period for both the first and the second implant of each
patient, defined respectively as the first 12 hr and the last 12 hr of the implant (Figure 2A). The
choice of 12-hr period probed acute and potentially transient post-implantation changes,
given evidence for changes in clinical seizures that may begin soon after implantation and
persist up to 3 days (Kovac et al., 2014). As a secondary analysis, we examined shorter periods
of 3 hr and 6 hr, as well as longer periods ranging from 24 to 60 hr in 6-hr steps (Bonferroni
correction for multiple periods in secondary analyses).
We compared the overall spike rate between the early and late implantation periods using a
paired t test across patients. We performed this analysis separately for three conditions: (a) the
original electrodes in the first implant, (b) the original electrodes in the second implant, and (c)
the added electrodes in the second implant. We next measured the relative change in spike
rate between the early and late period for each of the three conditions. We tested for a differ-
ence in the early to late relative spike rate change between the first and second electrode
implantation, as well as between the newly added electrodes and the original electrodes in
the second electrode implantation using two separate paired t tests across patients. Finally,
we tested whether patients with longer implant durations had a larger relative change in spike
rates. To do this, we measured the time between the beginning of the early period and the
beginning of the late period of each implant, and we calculated the Spearman rank correlation
across patients between early to late time difference and relative spike rate change.
Analysis of Electrographic Changes Pre- to Post-Implant Revision
We restricted subsequent analyses to the original electrodes that remained in place throughout
the entirety of recording, allowing us to directly compare pre- and post-revision features on
these electrodes. Our primary analysis defined a 24-hr peri-revision interval (12-hr pre-revision
and 12-hr post-revision, ignoring periods of electrode disconnection). We repeated this anal-
ysis for peri-revision interval durations of 6 and 12 hr, as well as longer durations from 36 to
120 hr, in steps of 12 hr (with Bonferroni correction for this secondary analysis).
Change in Electrographic Features Surrounding Implant Revision
We determined whether the peri-revision change in overall spike rate was larger than expected
for random times throughout the recording. We compared the change in overall spike rates
from pre-revision to post-revision against that obtained from 10,000 Monte Carlo iterations
drawing from the original dataset but using randomly chosen pseudo-revision times. For the
Monte Carlo iterations in this and in subsequent analyses, we added a peri-pseudo-revision
pause equal to the duration of paused recording or electrode disconnection surrounding the
revision. This pause prevented a bias wherein Monte Carlo iterations would otherwise com-
pare time periods in closer temporal proximity than the true peri-revision calculation. The
Monte Carlo p value was the proportion of iterations for which the absolute value of the
pseudo-revision spike rate change was greater than or equal to the true peri-revision spike rate
change (α = 0.005, Bonferroni correction for testing 10 patients). We next tested whether there
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Connectivity distribution:
Vector containing the average node
strength of each electrode. The
distribution of connection strength
across electrodes.
was a consistent difference between spike rates in the pre- versus post-revision periods across
patients using a paired t test (α = 0.05).
We next examined how the distribution in spikes across electrodes changed in the peri-
revision period. Separately for the pre- and post-revision periods, we defined the spike rate
distribution vector to be the average spike rate in each electrode in that period. We defined
the spike stability as the Spearman rank correlation between the pre- and post-revision spike
rate distribution vectors. Higher spike stability implies a lower change in the spike rate distri-
bution across electrodes between the pre- and post-revision periods. We used a Monte Carlo
test (10,000 iterations) to determine whether the peri-revision spike stability was lower than
expected for randomly chosen pseudo-revision times for any individual patient (α = 0.005,
Bonferroni correction). We performed Fisher’s combined probability test to aggregate p values
across patients (α = 0.05) (Fisher, 1934). A significant result implies that the peri-revision
change in spike rate distribution is larger than expected for randomly chosen times.
We also tested whether the distribution in functional connectivity changed in the peri-
revision period. We measured the node strength of each original electrode, defined as the
sum of the Pearson correlation coefficients across other original electrodes (Fornito et al.,
2016). We measured the average node strength in each electrode in each time period, defining
a connectivity distribution vector for each period. We defined the node strength stability as the
Spearman rank correlation between the pre- and post-revision vectors. We performed a Monte
Carlo test (10,000 iterations) to compare the node strength stability with that observed at ran-
dom pseudo-revision times (α = 0.005). We performed Fisher’s combined probability test to
aggregate p values across patients (α = 0.05).
Correlation Between EEG Feature Changes and Distance From and Connectivity To Implanted Electrodes
We hypothesized that changes in spike rate and functional connectivity would be larger for
original electrodes closer to or more connected to the site of implant revision. We examined
three proximity measures for each original electrode: the Euclidean distance to its nearest
newly implanted electrode, the average Pearson connectivity to the new electrodes, and the
co-spike index with the new electrodes.
We calculated the peri-revision relative change in spike rate and node strength between the
pre- and post-revision periods. We then measured the Spearman rank correlation between the
vector representing the peri-revision relative change in the electrographic feature and the vector
representing the proximity to the revision site (each vector was NEO × 1 in size, where NEO is the
number of original electrodes), defining the proximity-change correlation. Electrodes with zero
spikes pre-revision sometimes had an infinite relative increase in spike rate. This occurred for
an average of 5.3 (3.8%) electrodes across patients. Spearman correlation ranks these electrodes
as tied for the highest relative spike rate change. We performed a Monte Carlo test (10,000 itera-
tions) to test whether the proximity-change correlation was larger than expected for randomly cho-
sen pseudo-revision times (α = 0.005). We next tested whether there was a consistent direction of
correlation between the EEG feature and proximity measure across patients. We performed Fisher’s
r- to z-transformation on each proximity-change correlation to transform it to an approximately
normally distributed value z (Fisher, 1915). We aggregated the z values across patients and per-
formed a two-sided t test to determine whether these were significantly different from 0 (α = 0.05).
Anatomical Differences in Changes in Peri-Revision Spike Rate and Connectivity
We next tested whether different anatomical regions demonstrate different changes in peri-
revision spike rate or connectivity. This method is described fully in the Supporting Information.
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Effect of Electrode Type on Implant Effect
We asked whether electrode type—depth versus subdural—influenced the effect of electrode
implantation on spike rates and functional connectivity. We performed a limited within-patient
analysis on Patient 2 (the only patient with both depth and subdural electrodes added). We
measured the distance in millimeters between each original electrode and the nearest added
depth and subdural electrode, respectively. We identified those electrodes whose distance was
less than 20 mm (a threshold chosen based on visualizing a histogram of distances; we also
tested threshold distances of 10 and 30 mm). We compared the peri-implant relative change in
spike rate and node strength between those electrodes most proximate to added depth elec-
trodes and those most proximate to added subdural electrodes using a Mann-Whitney U test.
Statistical Analysis
All analyses were performed in MATLAB 2021a (Mathworks). The Skillings-Mack test was per-
formed using the skillmack package in MATLAB (Pingel, 2010). EEG data are publicly avail-
able on ieeg.org. All code, along with an intermediate dataset containing spike detections and
network calculations, is available on GitHub (Conrad, 2021; https://github.com/erinconrad
/interictal_hubs/tree/main/implant_analyses).
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ACKNOWLEDGMENTS
The authors thank Jacqueline Boccanfuso for her help curating EEG data.
SUPPORTING INFORMATION
Supporting information for this article is available at https://doi.org/10.1162/netn_a_00248.
AUTHOR CONTRIBUTIONS
Erin Conrad: Conceptualization; Formal analysis; Writing – original draft. Russell Shinohara:
Formal analysis; Supervision; Writing – review & editing. James Gugger: Validation; Writing –
review & editing. Andrew Revell: Visualization; Writing – review & editing. Sandhitsu Das:
Methodology; Software; Writing – review & editing. Joel Stein: Methodology; Software;
Writing – review & editing. Eric Marsh: Conceptualization; Writing – review & editing. Kathryn
Davis: Conceptualization; Supervision; Writing – review & editing. Brian Litt: Conceptualiza-
tion; Supervision; Writing – review & editing.
FUNDING INFORMATION
Erin Conrad, National Institute of Neurological Disorders and Stroke (https://dx.doi.org/10
.13039/100000065), Award ID: R25 NS-065745. Erin Conrad, National Institute of Neurolog-
ical Disorders and Stroke (https://dx.doi.org/10.13039/100000065), Award ID: NIH
1K23NS121401-01A1. Russell Shinohara, National Institute of Mental Health (https://dx.doi
.org/10.13039/100000025), Award ID: R01MH112847. Russell Shinohara, National Institute
of Neurological Disorders and Stroke (https://dx.doi.org/10.13039/100000065), Award ID:
R01NS060910.
Institute of Neurological Disorders and Stroke
(https://dx.doi.org/10.13039/100000065), Award ID: T32NS091006. James Gugger, American
Epilepsy Society (https://dx.doi.org/10.13039/100001454). Andrew Revell, National Institute
of Neurological Disorders and Stroke (https://dx.doi.org/10.13039/100000065), Award ID:
5-T32-NS-091006-07. Eric Marsh, Intellectual and Developmental Disabilities Research Cen-
ter (https://dx.doi.org/10.13039/100007857), Award ID: P50 HD105354 Project 8785. Kathryn
Davis, National Institute of Neurological Disorders and Stroke (https://dx.doi.org/10.13039
James Gugger, National
Network Neuroscience
847
Testing the implant effect
/100000065), Award ID: R01NS116504. Kathryn Davis, National Institute of Mental Health
(https://dx.doi.org/10.13039/100000025), Award ID: R01MH117155. Kathryn Davis,
Pennsylvania Tobacco Fund. Kathryn Davis, Thornton Foundation. Brian Litt, National Insti-
tute of Neurological Disorders and Stroke (https://dx.doi.org/10.13039/100000065), Award
ID: DP1NS122038. Brian Litt, National Institute of Neurological Disorders and Stroke (https://
dx.doi.org/10.13039/100000065), Award ID: 2R56NS099348-05A1. Brian Litt, The Mirowski
Family Foundation. Brian Litt, Jonathan and Bonnie Rothberg.
COMPETING INTERESTS
R. T. Shinohara receives consulting income from Octave Bioscience, and compensation for
reviewing scientific articles from the American Medical Association and for reviewing grants
for the Emerson Collective, the National Institutes of Health, and the Department of Defense.
S. Das is a consultant for Nia Therapeutics. J. M. Stein receives support from two sponsored
research agreements with Hyperfine Research, Inc., and he is a consultant for Centaur Diag-
nostics, Inc. K. A. Davis reports the following conflicts of interest: Eisai (research funding, advi-
sory board), Eton Pharmaceuticals (advisory board), GW Pharmaceuticals (consultant), SK Life
Science (advisory board), and Pfizer (consultant).
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